phosphate buffer saline pbs incubation Search Results


pbs  (JULABO GmbH)
96
JULABO GmbH pbs
Pbs, supplied by JULABO GmbH, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs solution  (Waters Corporation)
86
Waters Corporation pbs solution
Pbs Solution, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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x pbs  (LI-COR)
86
LI-COR x pbs
X Pbs, supplied by LI-COR, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphate buffer saline  (Sysmex Corporation)
90
Sysmex Corporation phosphate buffer saline
Phosphate Buffer Saline, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 mg brdu in pbs  (Becton Dickinson)
90
Becton Dickinson 2 mg brdu in pbs
PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with <t>BrdU</t> at 2 h before treatment with PTx or <t>PBS</t> (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.
2 Mg Brdu In Pbs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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catéter de 24g bd insytetm  (Becton Dickinson)
90
Becton Dickinson catéter de 24g bd insytetm
PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with <t>BrdU</t> at 2 h before treatment with PTx or <t>PBS</t> (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.
Catéter De 24g Bd Insytetm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mac16 cells  (Harlan Laboratories)
90
Harlan Laboratories mac16 cells
PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with <t>BrdU</t> at 2 h before treatment with PTx or <t>PBS</t> (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.
Mac16 Cells, supplied by Harlan Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs-isolymph  (Gallard Schlesinger)
90
Gallard Schlesinger pbs-isolymph
PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with <t>BrdU</t> at 2 h before treatment with PTx or <t>PBS</t> (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.
Pbs Isolymph, supplied by Gallard Schlesinger, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs-sk  (Agilent technologies)
90
Agilent technologies pbs-sk
PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with <t>BrdU</t> at 2 h before treatment with PTx or <t>PBS</t> (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.
Pbs Sk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbluescript sk ii (pbs) polylinker region sequence  (Agilent technologies)
90
Agilent technologies pbluescript sk ii (pbs) polylinker region sequence
PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with <t>BrdU</t> at 2 h before treatment with PTx or <t>PBS</t> (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.
Pbluescript Sk Ii (Pbs) Polylinker Region Sequence, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbs buffer  (Nature Biotechnology)
90
Nature Biotechnology pbs buffer
PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with <t>BrdU</t> at 2 h before treatment with PTx or <t>PBS</t> (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.
Pbs Buffer, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against pv  (Swant)
90
Swant primary antibodies against pv
PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with <t>BrdU</t> at 2 h before treatment with PTx or <t>PBS</t> (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.
Primary Antibodies Against Pv, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with BrdU at 2 h before treatment with PTx or PBS (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.

Journal: The Journal of Experimental Medicine

Article Title: GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions

doi: 10.1084/jem.20090866

Figure Lengend Snippet: PTx blockade of monocyte recruitment reveals that proliferating intimal cells accumulate in 2-wk lesions, and intimal cell proliferation is independent of monocyte recruitment. (a) Flow cytometry plots of peritoneal exudate cells harvested 24 h after i.p. thioglycollate injection of control and PTx-treated mice (percentages are indicated). Representative data from five mice per group studied in two independent experiments are shown. Additional details are provided in Fig. S2 . (b) Ldlr −/− mice fed a CRD for 2 wk were injected with BrdU at 2 h before treatment with PTx or PBS (C, control). Aortas were harvested 24 h (left) and 2 h (right) after BrdU injection, and BrdU + nuclei were enumerated. (c) Ldlr −/− mice fed a CRD for 2 wk were injected with PTx, and BrdU pulse labeling was performed 22 or 46 h later. Aortas were harvested after 2 h, and BrdU + nuclei were enumerated. In b and c, four independent experiments were performed at each time point, and means ± SEM were derived from four mice per group. *, P < 0.05 relative to C; **, P < 0.01.

Article Snippet: In BrdU labeling experiments, mice received a single 0.2-ml i.v. injection of 2 mg BrdU in PBS (BD).

Techniques: Flow Cytometry, Injection, Labeling, Derivative Assay